Pdf evaluation of cyclophosphamideinduced genotoxicity and. Although it has some tumour selectivity, it also possesses a wide spectrum of toxicities. It may be concluded that turmeric and myrrh are useful herbal remedies, especially for controlling oxidative damages and genotoxicity induced. Khatib na, gautam ghoshal, hashilkar nayana, joshi rk, taranalli ad. Cyclophosphamideinduced nephrotoxicity, genotoxicity, and. Ginseng has often been clinically used with cp in china, but whether and how.
The chemoprotective effect of chenopodium album extract was tested against the genotoxicity induced due to cyclophosphamide cpa in human lymphocytes using chromosomal abberations ca as a. Cyclophosphamide, an orally active alkylating agent, is widely used to treat a variety of malignant and nonmalignant disorders. Take cyclophosphamide at around the same time every day. Many commonly prescribed chemotherapy drugs such as cyclophosphamide cyp have adverse side effects including disruptions in taste which can result in loss of appetite, malnutrition, poorer recovery and reduced quality of life. Mar 15, 2017 cyclophosphamide interferes with oogenesis and spermatogenesis. Request pdf protective effect of boron on cyclophosphamide induced lipid peroxidation and genotoxicity in rats the aim of the present study was to evaluate the possible protective effect of. A cyclophosphamide cp induced tolerance system in mice that primarily consists of donor cell injection followed by cptreatment was found useful for inducing a longlasting allo or xenotolerance to various solid organs. Early increases in pulmonary mrna encoding procollagens and transforming growth factorbeta in mice sensitive to cyclophosphamide induced pulmonary fibrosis. Diabetes mellitus is a metabolic disorder characterized by elevated level of glucose in the blood. Cyclophosphamide cp is associated with significant pulmonary toxicity. Protective effect of boron on cyclophosphamide induced.
Egyptian sweet marjoram leaves protect against genotoxicity, immunosuppression and other complications induced by cyclophosphamide in albino rats volume 108, issue 6 gamal ramadan a1. Data of 1018 patients 725 women293 men treated with cyc were evaluated in this retrospective study. Large interindividual variability in the activity of the human liver s9 fraction leads to differences in drug induced genotoxicity, as observed from in vitro genotoxicity tests. Its prescribed for nonhodgkin lymphoma, hodgkin lymphoma, multiple. Fulminant fatal cardiotoxicity following cyclophosphamide therapy. Pdf interactive effects of saffron with garlic and. Cyclophosphamide induced a markedly different snv mutation spectrum than cisplatin, with the elevation primarily of t a and c t mutation numbers. Incidence of cyclophosphamideinduced hemorrhagic cystitis in chinese han population with autoimmune disease di fu 1, shanhui ye, chuyin xiao, yingying xie, jianquan gao1, liuqin liang2. The length of treatment depends on the types of drugs you are taking, how well your body responds to them, and the type of cancer or condition you have. The present study was aimed to examine and evaluate the genotoxicity and cytotoxicity induced by different doses of.
Cytoxan cyclophosphamide dosing, indications, interactions. Ginsenoside rg1 enhanced immune responses to infectious. Protective effects of essential oils as natural antioxidants. To the best of our knowledge, this is the first report of severe, nonviral, liver inflammation developing within 24 hours of administration of lowdose intravenous cyclophosphamide 200 mg.
Evaluation of dna interaction, genotoxicity and oxidative stress. However, the studies on its potential to induce toxicity in normal cell lines and in vivo system are limited and ambiguity still exists. Effect of interindividual variability in human liver. The basic mechanisms of its toxic effect are lipid. It is a prodrug that is converted to its active metabolites in liver.
Amelioration of the cyclophosphamide induced genotoxic damage in mice by the. Caution should be exercised when handling and preparing cyclophosphamide for injection, usp lyophilized powder, or bottles containing cyclophosphamide. Simultaneous treatment with these plants significantly reduced the genotoxicity induced by lead administration and the powerful protection was observed with 5% powdered turmeric. Prevention of cyclophosphamideinduced hepatotoxicity and. Read citrus extract modulates genotoxicity induced by cyclophosphamide in mice bone marrow cells, journal of pharmacy and pharmacology. Cadmiuminduced genotoxicity, cytotoxicity and lipid. Cytoxan cyclophosphamide for injection, usp cytoxan.
Clinical application of cyclophosphamide cp as an anticancer drug is often limited due to its toxicity. Hepatotoxicity may occur even after lowdose intravenous cyclophosphamide treatment. Evaluation of genotoxicity of euphorbia triaculeata forssk. Cyclophosphamide is the generic name of the prescription drug cytoxan, a chemotherapy drug for people with certain cancers. Reducing the side effects of cyclophosphamide chemotherapy in.
Mn analysis showed a dosedependent induction of micronucleated. Genoprotective and genotoxic effects of thymoquinone bioline. Herbal medicines remain an important component of the health care system. Lowdose cyclophosphamideinduced acute hepatotoxicity. Patients will have counselling and receive a patient information sheet from their nephrologist prior to their first infusion at. Therefore, we felt that it was crucial to conduct in vitro tests using an appropriate number of human samples to evaluate potential drug induced genotoxicity. First trimester exposure to cyclophosphamide for the treatment of cancer or lupus displays a pattern of anomalies labeled cyclophosphamide embryopathy, including growth restriction, ear and facial abnormalities, absence of digits and hypoplastic limbs. Hence, cyclophosphamide is probably the most widely used chemotherapeutic drug in veterinary medicine. Cyclophosphamide tablets, usp are for oral use and contain 25 mg or 50 mg cyclophosphamide anhydrous. Cellular mechanisms of cyclophosphamideinduced taste loss. Previous studies in mice found evidence that cyp has a twophase disturbance in taste behavior. The mechanism of cyclophosphamideinduced hepatitis is not clearly understood. In addition a brief overview is given of the literature on treatment of lupus nephritis. Systemic anticancer treatments may exert their own toxic effects or can aggravate adverse effects of other drugs.
Full text full text is available as a scanned copy of the original print version. Gadjeva, modulatory effects of total extract of haberlea rhodopensis against the cyclophosphamide induced genotoxicity. In the present study we evaluated the potential of ipriflavone against the cytotoxic and mutagenic effects induced by cyclophosphamide chemotherapeutic agent in bone marrow cells of mice, using the. Tribulus terrestris tt has been considered as a potential stimulator of testosterone production, which has been related with steroidal saponins prevailing in this plant. In the present study, coffee cf was evaluated for its protective effects against genotoxic damage and oxidative stress induced by the chemotherapeutic drug, cyclophosphamide cph. Many different natural antioxidants were found to alleviate the toxic effects of various toxic agents via different mechanisms. Cyclophosphamide induced sterility may be irreversible in some patients. The anticancer drug cyclophosphamide induces granulosa cell apoptosis and is detoxified by glutathione gsh conjugation.
Coffee mitigates cyclophosphamideinduced genotoxic damage in drosophila melanogaster germ cells article pdf available in drug and chemical toxicology 425. In the treatment of acute myeloid myelogenous, nonlymphocytic leukaemia, cyclophosphamide has been used as an additional drug for induction or postinduction therapy. Cyclophosphamide cyp as one of the widely used antitumor agents creates crosslinks and strand breaks in dna of many cells like germ cells 20. Therefore, patients with sle and the wish to have a baby should not be primarily. Efsa guidance documents but not as yet incorporated into eu legislation. This probably induces sinusoidal obstruction syndrome. Cytoxan cyclophosphamide for injection, usp cytoxan tablets cyclophosphamide tablets, usp description cytoxan cyclophosphamide for injection, usp is a sterile white powder containing cyclophosphamide monohydrate. Ginseng has often been clinically used with cp in china, but whether and. Cadmium induced genotoxicity, cytotoxicity and lipid peroxidation in allium sativum and vicia faba.
Handle and dispose of cyclophosphamide in a manner consistent with other cytotoxic drugs. Incidence of cyclophosphamideinduced urotoxicity and. We report a case of cyclophosphamide induced cardiotoxicity in a patient with normal cardiac functions before chemotherapy. Cyclophosphamide is an alkylating agent used in the treatment of several forms of cancer including leukemias, lymphomas and breast cancer. Effect of an lcysteine based oxovanadiumiv complex on oxidative stress and dna damage author links open overlay panel. Effect of euphorbia triaculeata extract on induction of chromosomal. It is one of the most potent immunosuppressive drugs available. Prevention of cyclophosphamideinduced hepatotoxicity and genotoxicity. Jan 29, 2012 cyclophosphamide cpm, an alkylating agent is used as an immunosuppressant in rheumatoid arthritis and in the treatment of several cancers as well. In the present set of investigation, antimutagenic effect of. Unlike anthracyclines, cyclophosphamide induced cardiotoxicity does not appear to be cumulative. The data indicate that there is no hard evidence to support the superiority of longterm i. Protection by turmeric and myrrh against liver oxidative.
Shortterm comparative study of the cyclophosphamide. Protective effects of boron on cyclophosphamide induced lipid peroxidation and genotoxicity in rats. Protective effect of extracts of teucrium polium and rumex crispus against cyclophosphamide induced genotoxic damage in human lymphocytescover lettermeta1answers article pdf available july. Cyclophosphamide induced sister chromatid exchange in mouse primary bonemarrow and spleen cells solerniedziela et al. Original article incidence of cyclophosphamideinduced. Genotoxicity assessment of a pharmaceutical effluent using four. Prophylactic efficacy of melatonin on cyclophosphamide. Cyclophosphamide interferes with oogenesis and spermatogenesis. Dailymed cyclophosphamide injection, powder, for solution. Cyclophosphamide induced fatal cardiotoxicity is a rare complication, occurring within 10 days of receiving cyclophosphamide. This study was designed to evaluate the effect of oral administration of ginsenoside rg1 on oxidative stress induced by cyclophosphamide in chickens. Many commonly prescribed chemotherapy drugs such as cyclophosphamide cyp have adverse side effects including disruptions in taste which can result in loss of appetite, malnutrition, poorer recovery.
Acute pneumocyte injury, polyadpribose polymerase activity, and pyridine nucleotide levels after in vitro exposure of murine lung slices to cyclophosphamide. Mammalian cells posses a defense mechanism of scavenging these ros by. The dual behavior of tq observed in this study is dosedependent and therefore its mechanism of action needs. Cytoxan tablets cyclophosphamide tablets, usp are for oral use and contain 25 mg or 50 mg cyclophosphamide anhydrous. An external file that holds a picture, illustration, etc. Cardiac toxicity is one of the lifethreatening complications of cancer therapy. Aegle marmelos, plant known for its medicinal importance since ancient times and every part of the plant has been used for curing different diseases. Cyclophosphamide is extensively metabolized by the hepatic cytochrome p450 system. In the present set of investigation, antimutagenic effect of aegle marmelos bark amb extract. Cyclophosphamide tablets, usp description cyclophosphamide for injection, usp is a sterile white powder containing cyclophosphamide monohydrate. Cyclophosphamide induced myelosuppression can cause leukopenia, neutropenia, thrombocytopenia associated with a higher risk of bleeding events, and anaemia.
The mechanism may involve direct injury to the endothelium by phosphoramide mustard, an active metabolite of cyclophosphamide. Caution should be exercised when handling and preparing cyclophosphamide for injection, usp lyophilized powder, or bottles containing cyclophosphamide tablets. Cytoxan cyclophosphamide for injection, usp cytoxan tablets. A 66yearold caucasian woman with a mediastinal mass diagnosed with burkitt. Cyclophosphamide is used in the treatment of acute lymphoblastic leukaemia, especially in children. Protective effect of boron on cyclophosphamide induced lipid. In this study, ellagic acid ea, a naturally occurring plant polyphenol, was evaluated for its antigenotoxicity and antioxidant efficacy against the cpminduced renal oxidative stress and genotoxicity in swiss albino mice. Scientific opinion on genotoxicity testing strategies applicable. In the different sptreated groups, cp genotoxicity was found to be reversed. Manual of vascular plants of northeastern united states and adjacent canada. In this study, ellagic acid ea, a naturally occurring plant polyphenol, was evaluated for its antigenotoxicity and antioxidant efficacy against the cpm induced renal oxidative stress and genotoxicity in swiss albino mice. Dna damage induced by alkylating agents and repair pathways. Fatal pulmonary toxicity after a single dose of cyclophosphamide.
Jul 14, 2014 cardiac toxicity is one of the lifethreatening complications of cancer therapy. Genotoxicity assays based on induction of dna damage response ddrstress. Cyclophosphamide sugarcoated tablets final degree project 5 abstract cyclophosphamide is a cytotoxic antitumor prodrug belonging to the alkylating agents which is indicated for the treatment of many cancers as well as some immunerelated. It is challenging to explain the mutation spectrum based on the available evidence of cyclophosphamide induced lesions. Therapeutic use of cyclophosphamide and its cytotoxic. Cyclophosphamideinduced nephrotoxicity, genotoxicity, and damage in kidney genomic dna of swiss albino mice. Cyclophosphamide cp, a chemotherapeutic agent, is restricted due to its side effects, especially hepatotoxicity. Aqueousethanolic extract of cassia alata aecal and its derived fractions obtained through liquidliquid fractionation were evaluated for their bronchorelaxant, genotoxic, and antigenotoxic effects. Oct 30, 2012 cyclophosphamide, an orally active alkylating agent, is widely used to treat a variety of malignant and nonmalignant disorders. Development of sterility appears to depend on the dose of cyclophosphamide, duration of therapy, and the state of gonadal function at the time of treatment. Antimutagenic activity of ipriflavone against the dna. Cyclophosphamide cp is widely used anticancer and chemotherapeutic drug 21. Get a printable copy pdf file of the complete article 822k, or click on a page image below to browse page by page.
The requirement of metabolic activation before cyclophosphamide. The requirement of metabolic activation before cyclophosphamide exerts either its therapeutic or toxic effects is well established, but has not led to effective countermeasures. Injury to 51crlabeled bovine artery pulmonary endothelial bpae cells was quantified by the release of 51cr from. Although alkylating agents do not directly induce dsbs, dsbs are detected in wildtype cells and other cell culture systems after the processing of dna lesions induced by alkylating agents. Cyclophosphamide cpm, an alkylating agent is used as an immunosuppressant in rheumatoid arthritis and in the treatment of several cancers as well. Genotoxicity and pharmacokinetic characterization of. Although not all of these side effects may occur, if they do occur they may need medical attention. To assess bladder toxicity of cyclophosphamide cyc and uroprotective effect of mesna in rheumatic diseases. We previously showed that both cyclophosphamide treatment and gsh depletion induced granulosa cell apoptosis in rats, but the role of gsh in apoptosis in human ovarian cells has not been studied. Pdf genoprotective effects of origanum vulgare ethanolic extract. Cp is metabolized mainly in the liver by cytochrome p450 system into acrolein which is the proximate toxic metabolite. A comprehensive survey of the mutagenic impact of common.
Ginseng alleviates cyclophosphamideinduced hepatotoxicity. In the present study, the protective effects of septilin against the genotoxicity of cyclophosphamide cp a widely used alkylating anticancer drug was evaluated by using in vivo micronucleus mn and sperm. Cytoxan tablets cyclophosphamide tablets, usp are for oral use and contain 25 mg or 50 mg cyclophosphamide. Apr 15, 2020 cyclophosphamide, a nitrogen mustard, is an alkylating agent from the oxazophosphorine group figure 1. Cyclophosphamide is a pregnancy category d drug and causes birth defects. Inactive ingredients in cyclophosphamide tablets are. Protective effects of fullerene c60 nanoparticles and. A read is counted each time someone views a publication summary such as the title, abstract, and list of authors, clicks on a figure, or views or downloads the fulltext.
Genoprotective effects of origanum vulgare ethanolic extract against cyclophosphamideinduced genotoxicity in mice bone marrow cells. Intravenous cyclophosphamide for renal patients protocol 1. Protective effect of boron on cyclophosphamide induced lipid peroxidation and genotoxicity in rats article in chemosphere 108 february 2014 with 229 reads how we measure reads. An in vitro endothelial model of injury was developed to assess the direct toxic effects of cp, cp derivatives and cp metabolites on cultured endothelial cells. Chemoprotective effect of spirulina arthrospira against. Hyperglycaemia perturbs the critical balance between oxidative stress and anti. Cyclophosphamide for injection, usp is a sterile white powder containing cyclophosphamide and is supplied in vials for single dose use. Cyclophosphamide can cause fetal harm when administered to a pregnant woman based on its mechanism of action and published reports of effects in pregnant patients or animals. Recently, much attention has been focused on the biological and medicinal properties of saffron. Saffron is a wellknown spice and food colorant commonly consumed in different parts of the world. Cyclophosphamide is a prodrug that is metabolised to active alkylating agents, principally by hepatic microsomal enzymes.
Pdf coffee mitigates cyclophosphamideinduced genotoxic. Pdf protective effect of extracts of teucrium polium and. Cyclophosphamide comes as a tablet to take by mouth once a day. On the other hand, cp has been shown to be genotoxic when given to females as well as males. In addition, the ethanolic extract was not able to neutralize clastogenicity induced by cyclophosphamide in treated rats but some attenuating effect on cyclophosphamide induced genotoxicity. In a study on cyclophosphamideinduced lipid peroxidation and gene toxicity in rats, boric acid was reportedly to reduce cyclophosphamideinduced oxidative stress in a dosedependent manner. The current study aimed to evaluate the protective effects of melatonin, a pineal secretory product, against hepatotoxicity induced by cyclophosphamide cp in mice. Lyophilized cytoxan cyclophosphamide for injection, usp. Along with its needed effects, cyclophosphamide may cause some unwanted effects. Although cardiotoxicity is related to dosage, previous anthracycline treatment, previous radiation, age over 50, and the presence of left ventricular lv dysfunction, no definitive risk factors have yet been identified. Follow the directions on your prescription label carefully, and ask your. Pdf evaluation of cyclophosphamideinduced genotoxicity. Tribulus terrestris protects against male reproductive.
Amelioration of the cyclophosphamide induced genotoxic damage. Cellular mechanisms of cyclophosphamideinduced taste loss in. Antigenotoxic effects of a polyherbal drug septilin. Therapeutic use of cyclophosphamide and its cytotoxic action.